Determination of cytotoxicity induced by 1,4 dioxane in root tip cells of Allium cepa L.(Amaryllidaceae)

In this study physiological and cytogenetic effects of 1,4 Dioxane used in daily lives frequently in the composition of cleaning and cosmetic products on Allium cepa L. root tip cells were investigated. A.cepa seeds was used as test material. Germination percentage, root lenght and weight gain was used as physiological indicators and chromosomal damage, micronucleus (MN) frequency, mitotoc index (MI) was used as cytotoxicity indicators and these datas were correlated with statistical parameters.The seeds of A.cepa were divided into three groups: control, two 1,4 Dioxane treatment groups, and they were treated with different two doses ( 50 and 100 ppm) of 1,4 Dioxane during 72 hours.As a result, it was determined that Dioxane fairly decreased the germination percentage, root length and weight gain depending on dose in seeds all treatment groups, whereas chromosomal damage and MN rate was increased. In conclusion, datas obtained in this study indicated that 1,4 Dioxane has cytotoxic effects a dose depending on root tip cells of A.cepa.