Kolon kanseri için pH bağımlılığı yoluyla seryum oksit nanopartiküllerinin farmakolojik potansiyeli

Nanoparticle-based cancer treatments have become attractive recently as an alternative to conventional treatments for colon cancer such as chemotherapy, biological agents, alkylating agents and antimetabolites. However, the major problem in traditional therapies is the side effects that arise in distinguishing normal and cancerous cells and resulting from complications that produce systemic toxicity. Therefore, the application of nanoparticles in cancer treatment emerges as a method aiming for controlled drug delivery and minimizing side effects. Unlike other nanoparticles such as silver, zinc, polymeric and gold nanoparticles etc., used to treat colon cancer, nanoceria is one of the most promising nanoparticles due to its excellent catalytic activities, which is derived from rapid oxidation state between Ce4+ and Ce3+. The hypothesis regarding the action mechanism of nanoceria is that cancerous cells are predicted to be acidic and nanoceria can increase the oxidative stress of these acidic cells and apoptosis leading to the destruction of cancer cells. In this study, after detailed characterization (UV-Vis, DLS, FTIR, Raman, XRD, XPS, TGA) of two synthesized Dex-CeNPs (SD1, SD2), their cytotoxicity, reactive species (ROS) scavenging properties and cell health status were evaluated in colon cancer cell lines. Cells were tested in cell culture at concentrations of 100, 250, 500 and 1000 ug/mL. The IC50 values of all cell lines were determined and SD2 synthesis was determined to be effective at lower doses between the two syntheses. However, flow cytometry experiments were continued over SD2 synthesis. With the high cytotoxicity observed in SD1 synthesis, UV-Vis and DLS characterization of the synthesis at pH 6 and pH 7 was performed. Two cell lines with relatively high ROS production (DLD-1 and COLO 201) compared to other three line was chosen for cytotoxicity assay at different pH values of tumors and healthy cells (6 and pH 7). In line with the results, it was observed that both syntheses were synthesized successfully. As a result of the high concentration requirement for SD1 synthesis, the concentration required for the IC50 value for DLD-1 at pH 6 decreased from the two tested cell lines. As a result of these sets of experiments, the concentration required for the IC50 of the SW48 line was greater than 5 mg/mL for both syntheses, so it was not used in the rest of the experiments. SD2 synthesis was promising for future experiments.