Determination of affects of feeding frequency on sperm and egg quality of Black Sea Trout (Salmo trutta labrax Pallas, 1811)

The aim of this study was to determine the effects of feeding frequency on sperm and egg quality of Black Sea trout (Salmo trutta labrax Pallas, 1811) in 2?3 years old. The study was performed by using 190 fish with a mean weight of 364.43±121.29 g. The sampled fish was counted and divided equally into two tanks. The tanks were named daily feeding frequencies of one (ff1) and two (ff2). Mean live weights of the fish in trial groups reached 847.39±238.47 g and 942.86±339.73 g at the end of the trial in groups, ff1 and ff2, respectively (p<0.05).Milt was collected by abdominal massage from 16 fish of the first group and 17 fish of the second group at the first stripping. At the first stripping, milt volume (ml), forward motility (%), the duration of the motility (sec), concentration (x109 cell/ml), sperm density (%) and pH values were 8.45±1.32 and 6.65±4.97, 76.67±18.53 and 87.36±9.63, 64.20±14.62 and 54.16±9.48, 21.40±7.08 and 23.40±5.51, 32.80±5.90 and 37.53±4.16, 7.17±0.16 and 6.94±0.09 for groups, ff1 and ff2, respectively. There were significant differences (the duration of the motility, sperm density, pH values and forward motility) between the groups (p<0.05). At the second stripping, milt volume (ml), forward motility (%), the duration of the motility (sec), concentration (x109 cell/ml), sperm density (%) and pH values were 3.55±2.06 and 2.27±2.22, 34.17±19.85 and 76.79±20.09, 45.05±8.35 and 47.55±8.80, 20.18±3.24 and 17.15±1.91, 32.07±3.94 and 32.81±3.56, 6.98±0.07 and 7.04±0.04 for groups, ff1 and ff2, respectively. There was significant difference (forward motility) between the groups (p<0.05).Egg was collected by abdominal massage from 26 fish for each group. At the first stripping, egg diameter (mm), egg weight (mg) and insemination rate (%) were 5.12±0.50 and 4.55±0.47, 81.95±13.02 and 76.48±10.24, 99.46±1.34 and 93.27±16.33 for groups, ff1 and ff2, respectively. There were significant differences (egg diameter and weight) between the groups (p<0.05).