Research for rifampicin and second generation antibiotic resistance genes in mycobacterum tuberculosis issues isolated at Duzce University between 2004-2021

ABSTRACT Background: The aim of our study is to determine the gene patterns that cause antibiotic resistance in M. tuberculosis complex strains isolated in our laboratory by molecular methods. Material and Method: In our study, 21 rifampicin-resistant M. tuberculosis complex strains identified between 2004-2021 and antibiotic susceptibility were determined were examined with the Genotype MTBC kit and the gene pattern causing rifampicin resistance with the Genotype MTBDR plus kit. Among them, the gene pattern causing second-generation drug resistance was examined with the Genotype MTBDR sl kit on 12 multi-drug resistant strains with rifampicin and isoniazid resistance together. Results: When the GenoType MTBC method of 21 rifampicin-resistant isolates was evaluated, 19 (90.4%) resulted as M. tuberculosis/canetti, two of which could not be evaluated with this test. Seven of them (36.8%) were resistant to rifampicin with Genotype MTBDR plus, while 12 (63.2%) were susceptible. In the resistant ones, deletion was observed in the WT8/WT6 region in one (14.3%) and in the WT8 region in one (14.3%), while one (14.3%) was deleted in the WT7 region with the rpoBMUT2A mutation and in four (57.1%) the WT8 region. deletion and rpoBMUT3 mutation was observed. While 7 (36.8%) of the strains were resistant to INH with Genotype MTBDR plus, 12 (63.2%) were found to be susceptible. Deletion and katGMUT1 mutation in the katGWT region was observed in five (71.4%) and inhAMUT3B mutation in two (28.6%) of the resistant ones. Genotypically, no resistance pattern was observed against any of the second-generation drugs in nine of the 10 multidrug-resistant M. tuberculosis isolates with the GenoType MTBDR sl ver 2.0 method. One strain could not be evaluated with this test. As a result, geotypic drug resistance may not always be observed in strains with phenotypic resistance. In our laboratory, the most common genotypic pattern for rifampicin was determined as rpoBMUT3 with deletion in the WT8 band. KEYWORDS: M. tuberculosis complex, Rifampicin resistance, Isoniazid resistance, Multidrug resistance