Investigation of antimutagenic effect of Rosa canina L. on root meristematic cells of Allium cepa L.

In this study, the antimutagenic effect of R. canina (rose hip) infusion against to mutagenic effect of the herbicide linuron was investigated on meristematic root cells of A. cepa (onion) by Allium test and flow cytometry method. In this context, firstly, roots of A. cepa were treated with 100 ppm linuron for 12 and 24 hours. Then the roots were recovered by R. canina infusion for 12 and 24 hours. In result of Allium test, depending on exposure time of linuron, it was determined linuron suppressed to mitosis in meristematic root cells and caused disturbed chromatin, sticky chromosomes, c-metaphase, chromosome bridges, vagrant chromosom abnormalities in meristematic root cells. In addition to these, linuron was found to inhibit of DNA synthesis in S-phase of cell cycle with flow cytometry analysis. It was found that the infusions of R. canina have activated DNA synthesis, arranged mitotic cell division and reduced chromosomal abnormalities of linuron. Thus, protective effect and healing the negative effects in mitotic cells of R. canina infusions against to the linuron toxicity on cell division was revealed.