Determination of brown trout gene expression after bacterial infection

In the present study, brown trout species (Salmo trutta labrax, S. t. caspius and S. t. abanticus) and their hybrids were exposed to Yersinia ruckeri causative agent of yersiniosis disease and Lactococcus garvieae causes lactococcosis diseases to test antioxidant and immune related genes expression. For this purpose, Catalase (CAT), Superoxide dismutase (SOD), Glutathione peroxidase (GPx), Glutathione reductase (GR), Cold-inducible RNA-binding protein (CIRBP), Cyclin-dependent kinase inhibitor 2A (CDKN2A), Prothymosin alpha 1 (ProT alpha 1), Transforming protein RhoA (RhoA), Immunoglobulin light chain (AL), Major histocompatibility complex class I (MHC class I), Hemoglobin subunit beta (HBB) and Stanniocalcin precursor (STC) gene of the fish were tested with EvaGreen based real-time PCR. After exposing fish to L. garvieae, oxidative stress genes and immunity related genes relative gene expression sometime down-regulated and sometime up-regulated in 14 days of post exposure (dpe). Furthermore, all relative gene expressions were downregulated after 14 dpe in all fish groups except S. t. caspius (CC). Although CC one of the most reacted fish after infecting with L. garvieae, Y. ruckeri infected CC was the least affected group. Therefore, gene expression was depending on fish and pathogenic bacteria.