The effects of some Schiff bases on some biochemical parameters in Saccharomyces cerevisiae cultural environments

Biochemical effects of newly synthesized some Schiff bases on living creatures need to be investigated before using them. For this purpose, Saccharomyces cerevisiae is involved in the most important cell model. Because metabolic properties of S. cerevisiae are similar to the highly organized organisms, the results show parallelism, as well. In the present study; lipid peroxidation (MDA), total protein, and glutathione analyses of newly synthesized Schiff bases were conducted in S. cerevisiae BY4741 culture media. For this purpose, YEPD medium was prepared for development and growth of S. cerevisiae BY4741 used in the experiment. 2ppm, 4 ppm, and 8 ppm from each of Schiff bases were added in to culture medium for application groups. GSH, total protein and MDA analyses were carried out with the obtained supernatant. When the results of lipid peroxidation were compared with the control group, it was determined that the schiff base groups had no lipid peroxidation inhibition effects, and the newly synthesized schiff bases were found to have significantly higher amounts of glutathione and total protein than the control group (p<0.0001). As a result, newly synthesized Schiff bases were determined to have different effects on biochemical and defense system of S. cerevisiae. In particular, the results of the antioxidant defense system will be a source for similar studies on other living models and will contribute to the knowledge of the literature as support for future studies using in vivo systems.