Biochemical study on the correlation of vitamin E and NOX2 levels in acute leukemia patients

Leukemia is the cancer of the body's blood-forming tissues, including the bone marrow and the lymphatic system. Acute Lymphocytic Leukemia (ALL) can be seen in adults but it is the most prevalent form of leukemia in children. On the other hand, Acute Myelogenous Leukemia (AML), seen in both children and adults, is the most frequent type of acute leukemia in adults. Vitamin E is the major lipid-soluble antioxidant in the cell antioxidant system. Reactive oxygen species (ROS) are highly reactive chemicals formed from oxygen, having potential to cause damage to the lipids, proteins, DNA and eventually result in cell dysfunction. The aim of this study was to search for a correlation between vitamin E and NOX2 levels in acute leukemia patients. During the period of February to June 2021, whole blood samples were collected from 45 acute leukemia patients (30 ALL and 15 AML) and 45 control subjects. Each serum sample was analyzed to measure vitamin E and NOX2 by Elisa assay. The median serum level of vitamin E was found to be 4.26 pg/ml in both patients (3.58-7.96 pg/ml) and the control group (3.12-22.96 pg/ml). However, the mean serum level of NOX2 in patients was found to be significantly high (130.17±21.88 pg/ml) compared to that of control group (121.74±13.91). Furthermore, a positive significant correlation was determined between Vitamin E and NOX2 in patients (r= 0.758, p<0.001). Key Words: Acute Leukemia, Vitamin E, NOX2, ELISA.