Isolation of lignin degrading bacteria from different sources and testing their ligninolytic activities

This study is about the isolation of the bacteria degrading lignin out of soil and fertilizer samples contaminated with petrol, and the identification of species. Eight different isolates were obtained from the soil and fertilizer samples, and according to the analysis, it was determined that L1, L2, and PT21 isolates were the different strains belonging to species of Enterobacter carcerogenus, L3 and L4 were different strains of Enterobacter ludwigii, PT22 isolate belonged to Citrobacter sedlakii, PT41 belonged to Citrobacter farmeri, and G1 isolate was the strain belonging to the species of Klebsiella pneumonia. The Kraft lignin degradation ratios of L1, L2, L3, L4, PT21, PT22, PT41 and G1 were identified to be 37 %,14 %, 20 %, 43 %, 48 %, 51%, 28%, and 60% respectively. The peroxidase was found in all isolates, whereas laccase was identified only in PT41 isolate. As the efficiency of isolates in waste dye removal was examined, they were observed to degrade RBBR at 20-90 %. Lignin peroxidase enzyme obtained from G1 isolate decolorized the dyes to the proportions between 19-70 % by using them as substrate. However, the enzymes derived from the other isolates were not efficient so much. The efficiency of the isolates in decolouring the direct dyes used for fabrics was examined. L1, PT21, PT22, PT41, and G1 isolates were observed to remove dye, and have decolourizing capacity. Ligninolytic enzymes obtained from microorganisms are used in paper, textile industries. In the light of the acquired data, the isolates were found to have sufficient capacity for industrial utilization. Based upon these findings, the enzymes to be obtained from isolates are thought to take their places among the industrial enzymes.