Effect of doxorubicin loaded microbubbles on mammary cancer in in-vivo experimental animal model

In-vitro effects of doxorubicin (DOX) and Doxorubicin confined to liposomes (Lipo-DOX) on 4T1 breast cancer cells were examined. MB images were displayed under ultrasound and demonstrated to be detonated under ultrasound in a controlled manner. Approximately 6 weeks of female athymic nude mice were used for the animal model. 5 experimental groups were formed. The number of animals in each group was determined as 7. When 4T1 cells were subcutaneously injected and the tumors reached 1cm in diameter, nude mice underwent 3-dimensional tumor volume measurement under ultrasound followed by drug administration. The drug-treated mice were sacrificed on the 4th day of the study, again following a 3-dimensional tumor volume measurement under ultrasonication. The tissues (lung, liver, kidney, brain, tumor) were stained with hematoxylin-eosin in sections after formalin fixation and histopathologically evaluated. The animals were also treated with 5 mg / kg DOX, which is a well-documented LD50 dose, in the breast cancer 4T1 cells. Lung, liver, kidney, brain and tumor tissues were examined histopathologically from tissues taken during sacrification of atymic nude mice. Normal histology was observed in all groups in the lung, kidney and brain. In the liver, in the DOX and Lipo-DOX groups, the damage in the spotty necrosis style was observed mildly in the parenchyma of 7 cases in each group. Necrosis rate, apoptosis rate, cell viability and liver damage were statistically significant when the tumor size difference did not give statistical significance between the groups (p = 0,145). As a result of our study, it has been shown that ultrasound contrast agent microtubules can be contrasted and controlled in vitro under in vivo conditions under ultrasound imaging. Doses of LD50 of DOX and LIPODOX have been shown to induce tumor necrosis in nude mice. In future studies, it is proposed to perform experiments with Doxorubicin loading at high doses of microbubbles, prolongation of microbubbles until sacrification to see if the microbubbles are detonated more than once under ultrasound and statistically significant decrease in tumor size. Keywords: Breast Cancer, Liposome, Doxorubicin, Microbubble, Ultrasonography