Dişki örneklerinde gastroenterit etkenlerinin araştirilmasi

Aim and hypotesis: Gastroenteritis is a major common worldwide public health problem. The fact that the complicating factors of gastroenteritis are determined and the treatment is determined depending on the results of antibiotic sensitivity testing is of great importance in improving mortality and morbidity, as well as in establishing the antimicrobical therapeutic regimen. The present study is aimed at determining the prevalence of major acute gastroenteritis, distribution by age and months, and resistance to antibiotics in those admitted to our hospital, and obtaining the data concerned as a basis for approaching the patient with gastroenteritis.Method: In the study was accomplished Laboratory of Department of Microbiology and Clinical Microbiology, Adnan Menderes University Faculty of Medicine, between 1th January 2007- 15th February 2008. We examined samples of stool from 200 subjects with the symptoms of gastroenteritis and 80 samples from those without gastroenteritis as controls, In the samples obtained, the species of Campylobacter, Aeromonas, Yersinia enterocolitica, EHEC, V. cholerae, Shigella, Salmonella were investigated using culture, and rotavirus and adenovirus antigens by immunochromatographic method. The strains of Campylobacter were identified using traditional methods (VIKIA Rota-Adeno, BioMérieux), API Campy commercial kit (BioMérieux), and polymerase chain reaction.Result: Campylobacter jejuni was found in nine (4,5%) and Salmonella spp. in five (2,5%) of the culture specimens from those with gastroenteritis, but no Aeromonas spp., Yersinia enterocolitica, Enterohemorrhagic Escherichia coli, Vibrio cholarae were detected. In this group we found antigens of rotavirus in 22 and adenovirus in three specimens. No fastidious organisms were found in any of the controls. Ofloxacin susceptibility of C. jejuni strains by E-test method was 56 percent, whereas no resistance to any other antibiotics tested was found.Conclusion: Rotaviruses, the most investigated among the gastroenteritis factors in our study, are the most common in children, as well as in adults. As C. jejuni is the most commonly detected bacterial agent, we believe that Campylobacter species must also be investigated in routine stool culture.Keywords: Campylobacter spp., Gastroenteritis, Salmonella spp., rotavirus, PCR.