The effect of curcumin on P53 /TIGAR and autophagy pathway in human osteosarcoma U2OS cell line

Osteosarcoma is a cancer disease from primitive bone tumor initiation and evolution of tubular long bones include femur, humerus and tibia. Osteosarcoma disease is defined and diagnosed throughout the inspection of tumor osteoblast cells that produce osteoid. Turmeric (Curcuma longa) is the most effective component in dried ground rhizomes of a member Zingiberaceae family. Curcumin have excellent free radical scavenging properties, however curcumin is almost the strongest antioxidant present in turmeric. Turmeric seasonal herbaceous plant with long lateral ramifications originally comes from Southeast Asia. Curcumin properties include anticancer, anti-bacterial, anti-inflammatory and antioxidant. Curcumin as chemical therapeutic agent demonstrated to suppress or inhibit various signaling pathways includes arrest tumor cell proliferation, inducing autophagy or apoptosis, inhibit malignant cell invasion and suppressing inflammatory responses. Despite the fact that effects of curcumin in cancer has been extensively studied, but the effect of curcumin on p53/TIGAR and autophagy has not been addressed in osteosarcoma U2OS cell line. In this study, we screened the weather of curcumin on human osteosarcoma U2OS cells after treatment, cultured cell were treated with various concentrations (10, 15, 20 and 25) µMolars curcumin intended for 24h. After cultured and treatment cells proliferation and viability were detected by MTT assay. The expression levels of these proteins (p53, TIGAR, LC3 and Beclin-1) were analyzed by immunoblotting assay and the expression of LC3 protein autophagy marker were analyzed by immunocytochemistry assay. The MTT assay results showed that curcumin caused distinct inhibition of osteosarcoma cell proliferation and cell viability. There were observed a linear dose/depends effect for curcumin range 15-25 µM dose cause to modulate of p53, TIGAR, Beclin1 and LC3. Analysed protein reveals a relation decrease in the levels of p53 and TIGAR proteins upon treatment with 10–25 µM curcumin. The result of immunocytochemistry showed that by increasing curcumin dose significantly increased the expression of the total amount LC3 protein in cytoplasm osteosarcoma U2OS cells. Our studied has improved the perceptive of the fundamental technique anticancer properties of curcumin. Keywords: Curcumin, U2OS, p53/TIGAR and autophagy.