Empirical and computational inquest into biosimilar and next-generation insulin analogs manufacturing: Unveiling structural, analytical, and dynamic attributes through recombinant technological approaches

The intricate molecular structure of insulin and its nuanced interactions within diverse tissues have carved out a domain within the scientific community dedicated to comprehending and harnessing the significance of this pivotal hormone. Investigative efforts on insulin encompass a broad spectrum of scientific disciplines, spanning cellular signaling, biochemical interactions, and genetic regulation. From this vantage point, insulin assumes a pivotal position at the nexus of modern biotechnology and fundamental science, constructing substantial contributions to the advancements in health and biological sciences. This thesis introduces a biotechnological product and method through the production and characterization of two new-generation domestic insulins but also offers the most comprehensive theoretical and analytical insights into the empirical and computational dynamics of these insulins and oligomeric commercial insulins. Introducing an atlas review encompassing all academic information about insulin, the thesis thoroughly explains the fundamental structural biology over this model protein. Subsequent chapters delve into the experimental and computational aspects of domestic new-generation rapid/ultra-rapid insulins. Furthermore, it presents revolutionary studies on the allosteric hierarchy of commercial long-acting oligomeric insulins. In this regard, this thesis can be regarded as a benchmark work in protein research