Isolation, molecular characterization and population analysis of Saccharomyces cerevisiae strains from sourdough samples collected from different province

In this thesis study, it was aimed to isolate Saccharomyces cerevisiae strains from 18 traditional sourdough samples collected from 3 different provinces (Aksaray, Niğde and Konya), and to conduct the genotypic characterization and population analysis using different DNA markers. In total, 58 of the 72 endogenous yeasts were identified as S. cerevisiae. In determining intraspecific genetic variation, SCoT 13 primer gave more useful results than iPBS and ISSR primers. As the distance between populations increased, the degree of genetic distance also increased (R = 0.74). The degree of genetic variation between populations (16%) and within populations (84%) was found to be statistically significant (P <0.001). Strains were divided into many subgroups on the UPGMA dendrogram, and according to STRUCTURE analysis, the number of significant subgroups was two (ΔK = 2). According to UPGMA and PCoA, clustering did not occur according to the regions where the strains were isolated, and random distribution was observed.