Hibridoma teknolojisi kullanılarak yeni apoptotik belirteçlerin tanımlanması

ABSTRACTIDENTIFICATION OF NOVEL APOPTOTIC MARKERSBASED ON HYBRIDOMA TECHNOLOGYCeren ÇıracıM.S. in Molecular Biology and GeneticsSupervisor: Assis. Prof. Tamer YağcıJuly 2004, 64 PagesApoptosis is a physiological cell death program that is characterized bymorphological changes such as nuclear condensation, membrane blebbing, cellshrinkage, DNA cleavage and formation of apoptotic bodies. It is required fornormal development and maintenance of cellular homeostasis anddysregulation of apoptosis leads to several developmental abnormalities,progression of degenerative diseases and cancer development. Elucidating themechanisms underlying this form of cell death and development of molecularmarkers for its identification are of great importance to understandtumorigenesis.One way to generate molecular markers is based on monoclonal antibody-production. Utilization of such antibodies as apoptotic markers is strictlydependent on their molecular specificity and selectivity in terms of recognizingtargeted proteins.We produced monoclonal antibodies by using apoptotic Huh-7hepatocellular carcinoma cells as immunogen, and chose two of them, namely9C11 and 6D5 for further characterization in the context of apoptosis.II