Memeli hücrelerinde Glypican 5'in hücre bölünmesi rolü

Glypicans (GPCs) are members of the Heparan Sulphate Proteoglycans (HSPGs) family of glycosaminoglycans. They are extracellular proteins anchored to the plasma membrane through their GPI anchor. Glypicans play role in developmental processes, cell differentiation, cancer progression, and stem cell function by regulating the activity of morphogens like Hedgehog and Wnt. An early RNAi screen has shown that the knockdown of Glypican 5 (GPC5) led to an increase in multinucleation suggesting a function in cell division. However, their role in cell division is not investigated. Our previous proximity dependent proteomic analysis in cytokinesis revealed that GPC5 interacts with proteins that are involved in cell adhesion, migration, actin reorganization, and RhoA activation. Even though cell division was extensively studied in terms of chromosome segregation and cytoskeleton dynamics, the function of cell surface proteins in cytokinesis remains elusive. In this thesis, I investigate the role of Glypican 5 in cell division by performing live cell imaging in GPC5 knock-out cells. In parallel, I investigated the functionality of previously identified interaction partners of GPC5. My analysis revealed that GPC5 have a role in the late cytokinesis by regulating adhesion properties of dividing cells specifically during abscission. This study illuminates the mechanism of how the outside and the inner part of the cell communicates during division.