Molecular identification and antimicrobial resistance profiles of Escherichia coli isolated in urine cultures from Shamiya State Hospital (Iraq)

In this study, Escherichia coli strains isolated from urine samples of patients admitted to Shamiya State Hospital (Iraq) were identified by conventional and automatic microbiological methods. Escherichia coli by percentage 29%, Staphylococcus aureus by percentage 21%, Klebsiella pneumoniae by percentage 18%, Proteus mirabilis by percentage 12%, Enterococcus faecalis by percentage 16% and Pseudomonas aeruginosa by percentage 4%. In our study, we used 11 Antibiotics (CFM, AM, AK, CN, CTX, ATM, CIP, LEV, IPM, TET, MRP) to show resistant, intermediate and susceptibility of Escherichia coli. The presence of Tet-A, Qnr-B, Ctx-M, TEM resistance genes in the identified E. coli strains was investigated by PCR. The CTX gene is a resistant gene compared to other genes in the total of bacterial isolates. The TET-A and TEM genes are a weakly resistant genes compared to the CTX gene. The QNRB gene is the weakest resistant gene compared to the CTX gene among the total isolates of bacteria. Demographic characteristics of individuals from whom E. coli strains were isolated were determined. The results obtained contributed to the development of clinical strategies for the prevention and management of E. coli infections causing UPEC in the Shamiya region.