Investigation of preventive effects of resveratrol against pancreatic dysfunction induced by polycyclic aromatic compound benzo(A)pyren in INS1-E (832/13) beta cell line

Diabetes is one of the most important problems in the world today. In all age groups and both sexes show a growing prevelans. This disease is a very important health problem due to affects many different organs and systems. According to data from the International Diabetes Federation, this disease affects the 8.3% of the world's population (382 million) and thi is estimated to be 592 million in 2035. The same organization suggests that approximately 183 million people is unaware of this disease. Environmental pollutants are known to be an important factor in many chronic disease. It has been well established that, environmental pollutants had deleterious effects on glucose metabolism, and caused insulin resistance and type 2 diabetes. With this investigation, it was aimed to investigate the effects of benzo(a)pyrene, a carcinogenic agent in so many organs or tissues such as lungs, skin, ovarium, colon, retina, liver and oesophagus, on pancreatic beta cells and treatment affects of resveratrol. In this study, rat pancreatic INS-1E beta cell line was used. After reaching the appropriate number of cells culture operations by cell culture, benzo(a)pyrene (20 µM, 24h) application have been made after resveratrol (80 µM, 48h) application. After incubations oxidative stress, insulin secretion (in cell and in medium), cell viability, cell proliferation and apoptosis were analysed in cells by biochemical and molecular techniques. B(a)P application resulted in NO increase and resveratrol also increased this level of NO. Resveratrol increaed the TAS levels decrased by B(a)P, and TOS levels were also increased by resveratrol interestingly. OSI levels determined with TAS and TOS levels, has no significant change between groups. GSH levels were decreased by B(a)P while resveratrol increased its' level to control level. mRNA expression levels of beta cell functions related genes INS-1, INS-2 and SIRT-1 were increased by resveratrol treatment. Insulin levels in cell and in medium were increased after resveratrol treatmant. mRNA expression level of FOXO-1 gene was increased while PDX-1 was decreased by resveratrol treatmeant. B(a)P suppressed the mRNA expression of p53 gene, but resveratrol increased. The effects of B(a)P on pancreatic beta cells and the protective effects of resveratrol on this cells were investigated in vitro with this research firstly. The results obtained from this research showed that oxidative changes, functional impairment and carcinogenetic effects of B(a)P in pancreatic beta cells could be blocked by resveratrol.