Investigation of methicillin, vancomycin resistance and panton-valentine leukocidin in staphylococcus aureus strains isolated from bovine mastitis

This study aimed to isolate the Staphylococcus aureus from bovine mastitis, investigate the mecA, vanA and pvl genes in isolated species by PCR and determine the antibiotic resistance of S. aureus strains to some antibiotics commonly used in veterinary field. In the present study, 602 mammary quarter milk samples belong to 170 lactating cows from 40 different small (family type) enterprises located center town and villages of Afyonkarahisar were used. CMT was applied to each mammary quarter. After the CMT scoring, the milk samples were aseptically collected from each mammary quarter. In this study, a total of 23 (%3,8) S. aureus strains were isolated from 602 mammary quarter milk samples by using a commercial identification kit. The presence of mecA, vanA and pvl genes was found in none of the strains. Phenotypic antibiotic resistances of all S. aureus strains to various antibiotics commonly used in veterinary field were investigated by using Kirby-Bauer disk diffusion method. The highest resistance rate in strains was against penicillin G (52.2%), followed by oxacillin (21.7%), erythromycin (21.7%) and amoxicillin+clavulanic acid (17.4%).