Clarifyingthe phenolic structure of bluebery (Vacciniummyrtillus L.) in Trabzon region using HPLC and determining the antioxidant proporties

Inthisstudy, it is aimedtoassessantioxidantproperties of blueberries (Vacciniummyrtillus L.) andtoclarifythestructure of biologicalactivecomponents of blueberriesbeingcollected Sürmene - Trabzon, in August of 2010. Fifteenphenoliccompoundswereanalyzedbyusingreversephase- highpressureliquidchromatography (RP-HPLC). Consequently 7 componentswerefound. Thesecomponentsandquantitieswere; syringicacid 7,53 mg/ 100 g DryWeight (DW) , chlorogenicacid 4,73 mg/ 100 g DW, benzoicacid 3,2mg/ 100g DW, protokatekuikaldehydeacid 1,64 mg/100g DW, cinapicacid 1,7 mg/100g DW, protokatekuikacid 0,89 mg/100g DW andvanillicacid6.7x10-4 mg/100 g DW.Besides, antioxidantactivities of blueberryextractsbeingprepared in fourdifferent, solventseach of which has ownspecificpolarityweredeterminedbyusingtheteststogetherwith total phenolic, total antosyanin, ferro (III) reductionantioxidantpower (FRAP) and DPPH (2,2-diphenyl-1-pikrilhidrazil) radicalcleaningactivity . Total phenolcontentwasdeterminedaccordingtostandard of gallicacid. FRAP valuestroloxequivalentantioxidantpower (TEAP), and DPPH radicalcleaningdeterminationresultswereexpressed as IC50 , alltheextractspossesedantioxidantactivity. Howewer in an order of methanolic>ethanolic>water>ethylacetate. A positivecorrelationwasestablishedbetweentheamount of phenolicsandthesolventtypestudied.