3 boyutlu (3D) basılmış pektin ve jelatin esaslı biyoaktif deri greftlerinin geliştirilmesi

The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not have proper viscosity and eliminated from printing studies. Based on the characterization studies, P1:G3 was chosen as the optimum composition. Curcumin (loaded as 100 or 150 µg/mL) release from the gels was higher at pH 7.4 than pH 5.0. The gels having higher curcumin demonstrated higher antibacterial effect on S.aureus but lower cell proliferation. For Vitamin-C (0.75 or 0.25 mM), spectroscopic detection was not succesful due to the interference of photo-crosslinker (Irgacure 2959). Therefore, the effect of Vitamin-C on collagen synthesis of fibroblasts was evaluated, where higher Vitamin-C containing gels generated higher collagen synthesis and enhanced cell proliferation. According to Live/Dead analyses, cell viability was above 80% for the samples having both bioactive agents upto 14 days. Bioprinted P1:G3 hydrogels containing L929 cells, curcumin and Vitamin-C resulted in high shape stability, viability and proliferation of the cells. The results indicated that curcumin and Vitamin-C containing P1:G3 hydrogels (either slab, printed or bioprinted) have high potential as skin graft and can be applied for the treatment of chronic wounds. In vivo studies are needed before any clinical application.