Bacterial isolation from Capnodis tenebrionis and determination of the insecticidal effects of these bacteria

Capnodis tenebrionis is one of the pests which create critical harm on the most of fruit trees from Coleoptera order. Therefore, struggle with these harmful pests is very crucial in both in our country and in the countries of Asia and Europe. The struggle with C. tenebrionis is done by insecticides. However, the alternative methods have gained importance because of insecticides's the damage of both nature and all creatures. Because of this reason, in this studying which aimed to strugle with this pest, 21 bacterial isolate from larvae and adults of C. tenebrionis's have been obtained and the morphological, physiological, biochemical and molecular properties have been defined. Due to this characterization studies, the bacterial flora of C. tenebrionis was defined as, Bacillus cereus (E-1, E-4, E-5, E-6, OL-4, L-2, L-5, L-6) Bacillus mycoides (OL-1), Bacillus pumilus (L-7), Paenibacillus xylanilyticus, (L-8), Bacillus flexus (L-9), Bacillus simplex (L-10, OL-5), Raoultella terrigena (L-1), Enterobacter cloacae (L-3), Bacillus anthracis (L-4), Klebsiella oxytoca (E-2), Bacillus safensis (E-3), Bacillus amyloliquefaciens (OL-2), Bacillus aryabhattai (OL-3). The insecticidal activities of the bacterial isolates on the larvae of the elm leaf beetle (Pyrrhalta luteola), the mealworm (Tenebrio molitor) and the honeycomb moth (Galleria mellonella) were investigated. It is detected that isolates with a number of OL-4, E-4 and E-5 have the highest lethal effect on these harmful larvae. These isolates are considered as an effective pest control factor.