Preparation of composite membrane embedded with Cu2+ attached O-carboxymethly chitosan Schiff base complexes and investigation for protein adsorption performance

In this study, Immobilized Metal Affinity Chromatograph (IMAC) finding a wide application area among affinity chromatography methods was used for removal of lysozyme efficiently from aqueous solutions. For this purpose, water soluble Ocarboxymethyl chitosan complexes was synthesised, Cu2+ ions was attached onto them. The composite cryogels were prepared by free radical polymerization using N,N,N´,N´-tetramethylene diamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair, N,N´-methylene-bis-acrylamide (MBAAm) as cross-linker in a plastic syringe of 5 mL. Prepared composit cryogel embedded with Cu2+ -attached Ocarboxymethyl chitosan Schiff base complexes was characterized by FT-IR, SEM, swelling tests measurements. The effect of pH, ionic strength, initial lysozyme concentration, temparature and flow rate on adsorption were investigated. The maximum amount of lysozyme adsorption from aqueous solution was at 103,3 mg/g particles in phosphate buffer at pH 8 with initial lysozyme concentration of 3 mg/mL. On the other hand, the most efficent adsorption was carried out at 0,5 mL/min, at 25oC temparature. It was also observed that enzyme could be repeatedly adsorbed and desorbed on the Cu2+ attached O-carboxymethyl chitosan Schiff base complexes croygels. Keywords: Lysozyme, Cryogel, Affinity, IMAC, Carboxymethyl Chitosan, Adsorption