The protective effect of dexpanthenol on experimental ischemia reperfusion injury of small intestine

The Protective Effect of Dexpanthenol on Experimental Ischemia Reperfusion Injury of Small Intestine PURPOSE: Purpose of this study is to investigate the protective effect of administration of dexpanthenole before and after creating ischemia and reperfusion injury in small intestine in rats. METHODS: 36 young and healthy rats (200-250 gr.) will be randomly divided into 6 groups. Group I: Sham Group II: Ischemia-reperfusion Group III: Given saline 30 minutes before ischemia then reperfusion for 60 minutes after 45 minutes of ischemia Group IV: Given 500 mg/kg of dexpanthenol 30 minutes before ischemia and then reperfusion for 60 minutes after 45 minutes of ischemia Group V: Given saline on 15th minute of 45 minutes of ischemia and then 60 minutes of reperfusion after ischemia Group VI: Given 500 mg/kg of dexpanthenol on 15th minute of 45 minutes of ischemia and then 60 minutes of reperfusion after ischemia All rats will be slept under anesthesia and their superior mesenteric arteries will be clamped using atraumatic microvascular clamp and thus ischemia will be created. Ileum specimens will be obtained and mucosal injury of small intestine according to Chius Score, loss of villi, grading of necrosis and inflammation and apoptosis using active caspase-3 will be investigated. Tissue malonyldialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), nitric oxide (NO), myeloperoxidase (MPO), reduced glutathione (GSH), glutathione reductase (GR) and glutathione peroxidase (GSHPx) activities and levels of blood interleukines has been also studied in similar small intestine specimens. All data will be evaluated using SPSS. FINDINGS: At least one group of rats was significantly different than others for MPO, IL-3 and Il-10 levels in specimens which were obtained after ischemia and therapy methods (p<0,05). There was no significant difference between groups in remainder of biochemical tests (p>0,05) but there was significant difference between some groups when considered in pairs (p<0,05). It has been established that scores of I/R groups were lower than therapy groups according to microscopic injury scoring in histopathologic examinations. CONCLUSION: It has been shown the protective effects of dexpanthenole against SOR in rat's testicle, eusophagus, small intestine and kidney in various I/R models. In our experimental ischemia reperfusion injury model of superior mesenteric artery, it has been shown that dexpanthenole which has been used before and after ischemia has protective effects correlated with MDA and IL levels and also leads to an increase in enzyme levels like SOD decreasing SOR injury. We think that administration of dexpanthenole for the first time for protection from ischemia reperfusion injury and correlation with bliochemical data is an important thing and we also think this may lead to further experiments. KEYWORDS: Dexpanthenole, experimental ischemia, reperfusion, small intestine, interleukin, necrotizing enterocolitis ADDRESS: Adnan Menderes University School of Medicine Pediatric Surgery Department AYDIN