Investigation of anticancer effect of disulfiram loaded nanoparticles on/in experimental breast cancer model

In this study, it was aimed to prepare a drug delivery system using magnetic silica nanomaterial (Fe3O4@mSiO2) which was non-toxic, biocompatible and porous. The drug-loaded structure was coated with folic acid linked polyethyleneimine (PEI-FA) to increase the water solubility of the drug delivery system and to provide specificity for cancer cells. Thus, a magnetic core mesoporous nano-carrier system (mMDPF) with ~160 nm dimensions was prepared. The drug-loading capacity of mMDPF was measured as 4.35%. Drug release kinetics of mMDPF was investigated and the best drug release was obtained at pH 6.0 and 37°C. The efficacy of the drug system in breast cancer treatment was improved by applying mMDPF with copper and/or sodium nitroprusside to MCF-7 and MCF-10A cells. It was observed that mMDPF was taken more by the MCF-7 cells and its toxicity on MCF-7 was much higher cells than MCF-10A cells.