The effect of different running treadmill exercise on oxidative stress parameters in rat muscle tissue

Regular exercises have increased antioxidant and vitamin requirements to reduce oxidative stress by reducing both the types of diseases caused by oxidative damage, improving overall quality of life and prolonging life, by improving both antioxidant defense and oxidative capacity in skeletal muscle. Physical exercises also lead to some changes in enzymatic antioxidant activity or non-enzymatic antioxidant concentrations. This study was planned to determine the effect of treadmill exercises made on different occasions on the oxidative stress parameters on the rat muscle tissue. In the present study, 32 male Albino Wistar rats weighing 280-350 grams were used. Rats were administered a treadmill exercise protocol for 13 weeks, short(15 minutes, n:8), modarate (30 minutes, n:8) and longtime(60 minutes, n:8) for five days. For each biochemical study, superoxide dismutase activity (SOD), malondialdehyde (MDA) levels and glutathione (GSH) levels from each supernatant were measured in duplicate in each rat muscle tissue by ELISA kits, respectively. Results from the experiments were given as mean±standard deviation, and P values below 0.05 were considered statistically significant. The significance level difference between groups was determined using the one-way analysis of variance (ANOVA) test and post-hoc tests using the "LSD" technique. The control group consisted of 23,07 ± 3,38 (U / mg protein), 15 min group (19,82 ± 4,77 U / mg protein) and 30,88 ± 4,18 (U / Running was determined as SOD value of 60 min 22.15 ± 3.85 (U / mg protein). The control group consisted of 0,43 ± 0,6 (nmol / mg protein), 15 minutes group with 0,35 ± 0,3 (nmol / mg protein), 30 minutes 0,23 ± 0,6 * ), Running 60 min 0,24 ± 0,1 (nmol / mg protein), GSH value. The control group consisted of 0.19 ± 0.02 (nmol / mg protein), 15 min group of running 0.12 ± 0.03 (nmol / mg protein), running 30 min 0.14 ± 0.01 (nmol / mg protein) , Running was 0,14 ± 0,01 (nmol / mg protein), GSH value for 60 min. As a result, it was determined that treadmill exercise performed at different times had a positive effect on the oxidative stress parameters occurring in rat muscle muscle. It may be suggested that the antioxidant supplementation taken with the exercises made at different times may be more effective on muscle tissue. Keywords: Rat, Treadmill, Exercise, Antioxidant, Oxidative Stress