Farklı tip propolis ekstrelerinin antiviral aktivitelerinin değerlendirilmesi

In this thesis, the effect of 4 different propolis extracts were investigated against Herpes Simplex Type 1 (HSV-1) and Herpes Simplex Type 2 (HSV-2) viruses. In-vitro cytotoxic effect of each extracts were determined by the Mitochondrial Dehydrogenase Enzyme Activity (MTS) method on Human Immortalized Keratinocyte (HaCaT) cell line and the cytotoxic concentration (CC50) values that killed 50% of the uninfected cells were calculated. The CC50 values were determined as 593 µg/mL, 375 µg/mL, 1723 µg/mL and 1664 µg/mL for the extracts of Propylene, Ethanol, Glycerol and Soya, respectively. Effective concentration (EC50) values that kill 50% of cells infected with HSV-1 and HSV-2 were analyzed by quantitative Real Time PCR method. The EC50 values calculated for HSV-1 were 86.64 µg/mL, 90.86 µg/mL, 768.6 µg/m L and 501 µg/mL for the extracts of Propylene, Ethanol, Glycerol, and Soya, respectively. The EC50 values calculated for HSV-2 were 92.05 µg/mL, 48.99 µg/mL, 904.1 µg/mL and 396.1 µg/mL for the extracts of Propylene, Ethanol, Glycerol and Soya, respectively. In vitro antiviral efficacy of an extract is determined by high of Selective Index values (SI:CC50/EC50). The SI values for HSV-1 were calculated as 6.84, 4.12, 2.24, 3.32 and 6.14 for the extracts of Propylene, Ethanol, Glycerol, Soya and Acyclovir, respectively. The SI values for HSV-2 were calculated as 6.44, 7.65, 1.90, 4.40 and 2.86 for the extracts of Propylene, Ethanol, Glycerol, Soya and Acyclovir, respectively. In comparison with the Acyclovir, Propylene extract showed significantly higher SI values while SI values for Ethanol, Soya and Glycerol extracts were lower than the values of Acyclovir for HSV-1. The SI values for Ethanol, Propylene and Soya extracts were significantly higher than the value of Acyclovir while no significant results were obtained for glycerol extract, in comparison with Acyclovir for HSV-2. As a conclusion it was revealed that in particular Propylene, Ethanol and Soya extracts of propolis having antiviral activity potantial against HSV-2.