Investigation of effects of nano-zinc oxide (ZnO) and nano-titanium oxide (TiO2NP) applications on some biochemical parameters of bean (Phaseolus vulgaris L.) plant

The simplest definition of nanotechnology is nanoscale technology. A nanometer is one billionth of a metre. Heavy metal stress generally induces ROS and creates oxidative stress. Substances such as antioxidant enzymes, metabolites, flavonoids, carotenoids, polyols, cytosolic ascorbate and peroxiredoxin play a role in ROS scavenging. Some antioxidant enzymes such as Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dimutase (SOD), Glutathione reductase (GR) defend against metal toxicity. In the present study, the effects of Titanium and Zinc nanoparticles on some biochemical parameters in bean (Phaseolus vulgaris L.) leaves were investigated. For this purpose, 0.1 mM, 0.01 mM and 0.001 mM TiO2 and ZnO nanoparticle concentrations were applied. At the end of 72 hours and 120 hours, malondialdehyde, proline, gutathion, total soluble protein, fatty acids, vitamins and phytosterol contents, and Superoxidedismutase and Catalase enzyme activities were determined. As a result, all the results obtained from the present study revealed that TiO2 NP and ZnO NP applications activate antioxidant defense in Pahseolus vulgaris L. leaves. It was determined that the two metal nanoparticles in question showed more serious effects, especially depending on the low dose and prolonged time. Nano-sized metals caused a toxic effect on the leaves of Pahseolus vulgaris L.