The effect of Fe2O3 nanoparticle on enzyme system of the greater wax moth Galleria mellonella larvea

In this work carried out, the effect of Fe2O3 (1, 2, 5, 10, 20, 50 μg/larva) nanoparticles given to stage 7th G. mellonella larvae by force-feeding method on carbohydrate, protein, lipid amount and oxidative stress markers (superoxide dismutase (SOD), catalase (CAT), melondialdehyde (MDA)) was determined by spectrophotometric method. The highest drop in protein quantity was 1.00 μg/Larva after 24 hours, despite the highest drop in the highest dose after 4 hours. Based on the level of the lipid amount, other doses were dropped outside of the dose of 1.00 μg/Larva after 4 hours and all doses were recorded after 24 hours. In the amount of carbohydrates, it was observed that there was a decrease in control five after 4 hours and 24 hours. Although the highest dose of the highest increase in the SOD enzyme flow after 4 hours, the maximum drop after 24 hours has been seen in the highest dose. CAT activity has decreased by control group of 5.00, 20.00, 50.00 μg/Larva doses after 4 hours, and other doses, except 1.00 μg/Larva, were seen at the end of 24 hours. An increase in the amount of MDA, one of the oxidative stress biomarkers, increased by control at the end of 4 hours, and an increase in the doses of 10.00,20.00 and 50.00 μg/Larva was observed at the end of 24 hours. As a result, in this study where iron oxide can have different effects on biochemical parameters depending on the dose and time shown using the model insect G. mellonella.