Synthesis of Fe3+ attached nanoflowers and investigation of specificity of them for DNA

Selection of purification method and type of adsorbent has high significance for separation of a biomolecule like deoxyribo nucleic acid (DNA). Nanoflowers are a newly improved class of adsorbent. Due to showing very structural similarity to plant flowers, they are named as nanoflowers. Herein, after synthesize ofcopper phosphate three hydrate nanoflowers [(Cu3(PO4)2.3H2O), CP-NFs], Fe3+ ions were attached to their surfaces. Obtained Fe3+-CP-NFs, before investigation of some adsorption parameters for DNA, they were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR). Some attained data from the results of adsorption experiments as follows: maximum DNA adsorption on Fe3+-CP-NFs was found as an excellent value like 845.8 mg/g with an initial concentration of 1.5 mg/mL DNA, at pH 7 and 25 °C. Langmuir and Freundlich adsorption equations were applied to determine which adsorption model was appropriate, and it was seen that Langmuir model was fit with a R2 of 0.9885.