Bor nitrür tabanlı nanomalzemelerin doku mühendisliği yapı iskelelerinde kullanımlarının araştırılması

Tissue engineering, a promising and an interdisciplinary field of regenerative medicine, aims to develop solutions by mimicking tissue/organ using natural, synthetic or semi-synthetic materials within the required functionality for the recovery/regeneration of lost or damaged tissue or organs. The currently used scaffolds in tissue engineering have inadequacies to be applicable due to need of more physical, chemical and biological enhancements to provide better cell adhesion, penetration, growth and regeneration. At this point nanotechnology idea comes into stage by using nanomaterials that have significant contributions to tissue engineering applications as reinforcement material so far. Nanomaterials can be preferred to manufacture high-performance biomaterials with tailored physical, chemical and biological features due to their superior mechanical, physical and chemical as well as greater biocompatibility properties. In this study, first, a novel and non-toxic piezoelectric composite, which include both barium titanates (BaTiO3) and boron nitride nanotubes (BNNTs) was aimed to develop to be used in biomedical applications. Then, three nanomaterials, barium titanates (BaTiO3), BNNTs and the novel composite were used by embedding into poly(Ɛ-caprolactone) (PCL) and chitosan polymers due to their high piezoelectric properties to introduce piezoelectric feature to the composite scaffolds. Electric-responsive Human Osteoblast cells (HOb) cells on scaffolds were stimulated by applying low-frequency ultrasound (US) stimulation during cell growth. Biocompatibility, cell adhesion, alkaline phosphatase activities and mineralization of HOb cells on piezo-composite scaffolds were investigated. Utilization of these piezo-composites scaffolds will be helpful for wireless tissue stimulation studies using piezoelectric phenomena in electrically sensitive cells or tissues to decrease the conventional invasiveness of in vivo electric stimulation.