Influence of oxidative biomarkers on ape1 gene expression in Iraqi breast cancer women

This study aimed to investigate the APE1 gene expression with some clinical biomarkers in breast cancer women. Ninety women were enrolled in this study; they were divided equally into 2 groups: the breast cancer patients' group, which attended the Al-Eluia hospital for women's care in Baghdad, and the healthy control women's group. Five milliliters of blood were taken from patients and a healthy control group. Two milliliters of blood were transmitted directly to EDTA tubes, and then the ELISA technique was used to determine the oxidative biomarkers like Glutathione Peroxidase 1 (GPX1), Estradiol (E2), Malondialdehyde (MDA), and reactive oxygen species (ROS). Three milliliters of blood were used for gene expression by using the RT-PCR method. The result of this study showed that there were significant differences in the expression of the APE1 gene were the gene folding for patients was 3.004 ±0.15 when compared with the control group 1.00 ±0.00, as for Estradiol was found elevated for the patients group the difference was highly significant when in breast cancer patients 146.26 ±10.07 when compared with the control group 34.19 ±1.80 the P-Value was 0.0001, glutathione peroxidase 1 (GPX1) were found elevated for the control group the difference was highly significant when in breast cancer patients 509.38 ±37.78 when compared with control group 1253.94 ±40.09 the P-Value was 0.0001, reactive oxygen species (ROS) were found elevated for the patients group the difference was highly significant when in breast cancer patients 623.19 ±67.53 when compared with control group 351.01 ±23.35 the P-Value was 0.0003 and in malondialdehyde (MDA) were found elevated for the patients group the difference was significant when in breast cancer patients 4.09 ±0.52 when compared with the control group 2.76 ±0.42 the P-Value was 0.050. In conclusion, when comparing breast cancer patients to healthy people, it was discovered that several factors (like GPX1, E2, MDA, ROS, and APE1 gene) were out of the ordinary.