Kemik doku mühendisliği için doğal kökenli silika takviyeli çift fiberli matrisler

Graft therapy is used to treat bone tissue loss, which has drawbacks; donor scarcity, risk of disease transmission and immune reaction. Tissue engineering scaffolds can overcome these drawbacks. In this study, a 3D scaffold that will support tissue regeneration at defect site was developed using mainly natural materials. Scaffold was produced by co-electrospinning and had two fiber phases; first phase was produced from a bacterial origin polymer, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and small amount of Polycaprolactone to support fiber structure, second phase was formed by pullulan (PUL) and diatom silica shells (DS) from single cell algae origin. Also, in the study, PHBV production by Cupriavidus necator bacterial strain was optimized and used to produce scaffolds. PHBV/PCL/CA:PUL/DS scaffold group was produced as dual fiber matrix and new crosslinking method for PUL was developed for crosslinking through electrospinning. Cefuroxime Axetil antibiotic was loaded into hydrophobic PHBV fibers. Characterization studies revealed that, in aqueous environment scaffold degrade slowly, take less water and has stable structure, support controlled release of antibiotic and improved compressive strength due to incorporated DS and double fiber structure. In vitro studies revealed that DS bearing groups improved Saos-2 cell viability and co-electrospun groups that have hydrophilic PUL fibers supported L929 cell viability. Scanning electrone microscopy and confocal laser scanning microscopy analyses showed that cells distributed through co-electrospun scaffold with healthy morphology. In the study, a novel, 3D, dual fiber scaffold was produced with natural origin base materials and has potential to be used for bone tissue engineering applications.