Effect of supermagnetic iron nanoparticular prepared with curcumin on oxidative stress on H2O2 induced SH-SY5Y neuroblastoma cell lines

In this study, 4 different curcumin iron nanoparticles (Kur@FeNP) in different molarities were synthesized by green synthesis method using curcumin active ingredient. Since Kur@FeNP1 has the best magnetic property, the studies continued on the characterization of Kur@FeNP1. The structural, morphological and optical properties of these NPs were characterized using UV-Vis, FTIR, XRD, SEM, TEM, EDX and Zeta potential. Although the sizes of the synthesized nanoparticles vary, it was determined to be 18 nm in average. Appropriate dose concentrations were determined in the neuroblastoma (SH-SY5Y) cell line to determine the neurodegeneration (H2O2) of Kur@FeNPs in vitro. Cytotoxic value was determined at 300 μM H2O2 concentration (LD50). In order to determine the effects of Ku@FeNPs on cell viability in the presence of H2O2, SH-SY5Y cells were treated with Ku@FeNPs in the concentration range (250-1.90 µg/ml) in the presence of LD50 (300 µM) H2O2. It has been observed that all these concentrations provide a neuroprotective effect against 300 μM H2O2. However, (Emax) Ku@FeNPs appear to have the most effective neuroprotective effect against oxidative stress-induced neurodegeneration at 250 μg/ml. This indicates that it has the capacity to strongly detoxify H2O2. TAS and TOS commercial kits were used as oxidative stress markers against H2O2 in the cell line. In antioxidant activity (TAS) studies, the highest increase in Kur@NPs in SH-SY5Y cell line compared to other group concentrations was Kur@NP1 250 µg/ml, Kur@NP2 250 µg/ml, Kur@NP3 250 µg/ml, Kur An increase in antioxidant levels was observed at the concentrations of @NP4 250 μg/ml and Curcumin 250 μg/ml. When the total oxidant status of SH-SY5Y cell lysates is examined (TOS); Among the groups treated with H2O2, it was determined that Kur@NP1 250 μg/ml nanoparticle showed the greatest decrease in total oxidant level.