The effects of oleuropein and cisplatin on neuroblastoma cells

Neuroblastoma (NB) accounts for 8-10% of all childhood cancers. Cisplatin (cis-diaminedichloroplatinum II; CDDP) is a chemotherapeutic drug with side effects that are used in the treatment of many different human cancers in childhood and adulthood. Oleuropein (OLE), an olive leaf, has been shown to reduce cell viability in cancer cells such as mesothelioma and malign melanoma. The aim of this study was to investigate OLE alone and in combination with CDDP has possible anti-proliferative effect and induce apoptotic cell death in good prognostic SH-SY5Y N-MYC (-) and poor prognostic KELLY N-MYC neuroblastoma and fibroblast cells. OLE (500uM) and CDDP (50uM) were applied to PSI2 12S6, SH-SY5Y and KELLY cells. Apoptotic cell deaths were determined by Akım cytometry using CDDP, OLE and a combination of OLE-CDDP. The anti-proliferatif and apoptotic effects of OLE on PSİ2 12S6 fibroblast cells were determined. OLE and CDDP-OLE combinations reduced viability in SH-SY5Y and KELLY cells and showed apoptotic effects. The mechanisms of anti-proliferative effects of OLE in neuroblastoma should be evaluated in vitro and in vivo studies.