Çift katman-elektro eğirilmiş iskeleler üzerine ekilmiş mezankimal kök hücrelerin osteojenik farklılaşması

Bone is the main component of the skeletal system in vertebrates. It provides mobility, locomotion and mechanically supports the soft tissues. Bone related diseases and injuries are generally caused by trauma, genetic malformations, bone loss/overgrow, infections and tumors. While simple fractures are easy to treat, complex fractures and pathological disorders entail arduous treatments. Current orthopaedic solutions encompass invasive surgical procedures and bone grafting (autografts, allografts and xenografts). Despite the fact that they are approved and commonly used methods, they have some unignorable disadvantages. Scientists sought alternative ways to overcome these drawbacks and conclude that tissue engineering could be promising treatment method for bone injuries. In bone tissue engineering, the scaffolds are combined with cells and biological stimulators to favor osteoconduction and osteoinduction during repair and regeneration of damaged or diseased bone. In this study, PCL (poly-ε-caprolactone)/PLLA (poly L-lactic acid) double layer nanofibrous electrospun scaffold system was designed to mimic natural extracellular matrix (ECM) of 3-D bone tissue. Bone morphogenetic protein-2 (BMP-2) loaded gelatin microspheres were embedded between two layers of electrospun scaffolds to favor osteogenic differentiation. In order to test the system, human adipose derived mesenchymal stem cells (hADMSCs) were seeded on the scaffolds. MTS cell viability assay showed that the double layer scaffold system supported the attachment and proliferation of the cells. The osteoinductive capacity of double layer scaffold system has been proven by alkaline phosphatase (ALP) assay, Von-Kossa staining and confocal microscopy analysis The result of these studies demonstrated that our scaffold system was suitable for the attachment, proliferation, differentiation and maturation of the hADMSCs.