Ozon ön arıtımının iki aşamalı anaerobik çürütücülerdeki biyogaz üretimi ve mikrobiyal konsorsiyum üzerindeki etkisi

The amount of sludge produced in treatment plants is increasing depending on the population growth and water consumption. Anaerobic digestion (AD) is a widely used sludge stabilization method in wastewater treatment plants, as it provides volume reduction and methane-rich biogas production. In single-stage anaerobic digestions, the process fails due to volatile fatty acid accumulation and pH changes. To solve this problem, two-stage anaerobic digestion (TSAD) process has emerged through physical separation of methane production stages. Various pretreatment applications are also used in AD to accelerate the rate limiting stage, hydrolysis. Of these, with its strong oxidizing ability ozonation stands out. Therefore, in this study, the effect of ozone pretreatment on TSAD was evaluated in terms of biogas production and microbial population dynamics. For this purpose, two-stage anaerobic digesters were operated after treatment with previously determined ozone dose of 0.06 g O3/g TSS and monitored in terms of pH, chemical oxygen demand, total nitrogen, solids content, total volatile fatty acids, biogas/methane production and microbial community structure. Biogas and methane production were monitored through water displacement method and gas chromatography, microbial consortium was monitored through fluorescent in situ hybridization method. Methane and biogas productions were found to be increased 69% and 38%, respectively. This increase was accounted for the dominance of Methanosaeta spp.