Investigation of the effects of cyclophosphazene derivatives on preventing lipid peroxidation on yeast profile

Cyclophosphazene-derived compounds are widely used in many fields such as industry, chemistry, biology, medicine and pharmacy. Our study aimed to determine the effects of four cyclophosphazene-derived compounds on yeast cells. YEDP medium was prepared for the development and proliferation of the S. cerevisiae yeast cell to be used in the experiment. Experimental groups were created in the medium environment, including a control group and 2, 4 and 8 µl concentration groups of the cyclophosphazen compounds used. After the sterilization process, each group of culture samples, which were brought to a temperature suitable for laboratory conditions, were inoculated with the culture of S. cerevisiae cells. After inoculation, it was left for incubation. Cells were measured according to cell density and collected by centrifugation. Total protein measurements were made by applying the Lowry method to Tris HCl-base and EDTA supernatants. Biochemical analyzes of lipid peroxidation (MDA), glutathione (GSH) and oxidized glutathione (GSSG) amounts were performed using the appropriate method on the HPLC device. Fatty acids were converted into methyl esters using Christe's highly efficient and practical acid-catalyzed esterification method and then analyzed in a gas chromatography device. As a result; the differences in MDA, Total Protein, GSH, GSSG and fatty acid levels in the groups to which the cyclophosphazene compounds were applied compared to the control showed that these newly synthesized cyclophosphazene compounds have various effects on the biochemical system and defense system of S. cerevisiae.