The effects of silostazole and pentoxifilina medicine on rat aorta endothelial functions in vitro with hypoxic and normoxic conditions

Aım The results of vasoconstriction in the pathophysiology of many diseases characterized by circulatory disturbances and consequent perfusion defects due to this disorder. Endothelium-releasing mediators, disorders that occur in the release of these mediators play a role in the development of peripheral artery disease. Because of the vasodilators and anticoagulant effects they have, pentoxifylline and cilostazol are used in a wide range of ischemic diseases, including peripheral arterial diseases and sepsis. In this study, the effects of cilostazol and pentoxifylline on contractile responses in vitro in rats were evaluated. Materıals And Method The weight of our study is 350-450 mg. 48 male Sprague-Dawley rats were used. The rats were divided into 6 groups. Each aortic segment was cut into a 3 mm ring. The Krebs solution was suspended without time. The two hooks were attached to the transducer at the top to measure the contractions in mg. The standard Equilibrium phase was then applied to each aortic ring. In normoxic condition, 8 of the rings were examined for contraction and relaxation curves without adding any drug. Cilostazol was added to the 8 rings in this ring and the relaxation responses of the rings were evaluated by adding 20% of the pentoxifylline to 8 rings. Twenty-four rings in hypoxic condition were allowed to stand for 20 minutes, then 8 of the rings were examined for contraction and relaxation curves without the addition of any drug. Cilostazol was added to the 8 rings in this carbonized krebs solution and the relaxation responses were evaluated by adding 20 pounds of pentoxifylline to 8 rings. Results Relaxation responses were obtained in groups of pentoxifylline and cilostazol in the aortic rings hanging in normoxic environment. In the normoxic medium on the endothelium, the relaxation response with cilostazol was significantly (p: 0.044) (p <0.05). However, the relaxation response with pentoxifylline on the endothelium in the normoxic medium was not significant (p: 0.149, p> 0.05). In the hypoxic 34 environment, the response of the cilostazol on the endothelium was p: 0.214 (p> 0.5). Concentration of pentoxifylline on the endothelium in the hypoxic environment was 0.000 (p <0.05) significant. The NO value of the aortic ring in the normoxic environment was lower than that in the normoxic environment. The decrease in this NO value in the cilostazol-administered group was significantly determined. An increase in NO was detected in the aortic ring in the pentoxylin-treated group in the hypoxic environment, and this increase was significant. Conclusıon In our study, relaxation response was obtained in both groups given cilostazol and pentoxifylline in normoxic environment. However, only relaxation response with cilostazol (p <0.05) was found to be significant. In the hypoxic environment, cilostazol and pentoxifylline were used to induce the contractile response and no relaxation response was obtained from both groups. However, this contraction response was considered significant for pentoxifylline (p <0.05).