Tasarım ve karakterizasyonusürekli glikoz izleme sensörü

The main objective of this thesis is the development and the elaboration of a lowcost continuous glucose monitor. This thesis describes the design of miniaturized continuous glucose monitoring sensor based on two needles. The sensor is based on enzymatic detection of glucose. The used enzyme is glucose oxidase entrapped inside a protecting film of chitosan. Chapter I provides a general introduction to diabetes and the different types of biosensors used for continuous glucose monitoring. "Continuous" monitoring approaches involve subcutaneous implantation of a micro-needle allowing real-time glucose level measurements in the interstitial fluid. The working principals of continuous glucose monitors (CGM)s are introduced. An overview of electrochemical sensors is presented, and the different methods of common electrode modification are also discussed in this chapter. The electrical circuits that are used for glucose sensors are reviewed. The second chapter contains the methods and materials used in the performed experiments. It describes the steps of sensor elaboration from the electrical circuit assembly on the PCB board to the electrode modification process. The measurement setup is also presented in this chapter. Chapter 3 discusses the results obtained for the sensor tests. Chapter 4 is the conclusion, it stipulates that after the tests carried on the elaborated biosensor both in the buffer solution and in the real sample, the sensor can be improved and it has potential to be used in tests on humans.