Triticum Aestivum Lin(Buğday Çimi) ve Mentha Piperita(Nane) kaynaklı eksozomların insan adipoz kök hücrelerinin adipojenik farklılaşması üzerine etkileri

In living organisms, when food intake is excessive, this excess energy must be stored, and multicellular organisms have specialized cells or organs to store this excess energy as lipids. The enzymes, hormones, growth factors, and cytokines secreted by adipose tissue play a vital role in the regulation of biological activities and energy metabolism in animals. Adipogenesis is defined as the transformation of preadipocytes into mature fat cells. In this study, adipogenesis was done by using human-derived adipose stem cells ordered from ATCC, and adipogenesis differentiation of these cells. In order to see whether they play a role in cellular fat breakdown, the exosomes isolated from Triticum aestivum Lin and Mentha piperita were characterized by size analysis on the NTA device. In order to determine whether exosomes have a toxic effect on human adipose stem cells, the MTS cytotoxicity experiment was carried out over six doses, and as a result, the experiments were continued by choosing a optimum dose. The optimum dose effect of exosomes from peppermint and wheatgrass on adipogenically differentiated cells were observed with Oil-Red-O staining. Adipogenic markers and their expressions in obesity markers were examined in the cells treated with mint exosome, in addition to these, the results were supported by ELISA and immunocytochemistry experiments. In line with these results, it was observed that peppermint exosome upregulated adipogenesis and induced obesity in cells treated with it. In addition, in cells treated with wheatgrass juice exome, it was observed that it downregulated adipogenesis and played a supportive role in the destruction of intracellular fat, as seen in the result of Oil-Red O staining. The findings of this study are encouraging for further research into the possible positive impacts of plant exosomes on adipose biology. In this sense, our findings guide for future research into plant exosome-based chemicals for the prevention and treatment of obesity, a modern pandemic with a strong relationship to comorbidities that is common globally.