Asymmetric reduction of prokiral ketones with Weissella paramesenteroides biocatalyst

Enantiomeric pure substances are one of the fields of interest of today's researchers in the industry. Chiral alcohols obtained in enantiomeric purity have a great place and importance in industry. As a result of the reduction of prochiral ketones to enantiomerically rich secondary alcohols, studies on the production of chiral substances with enantiomeric purity have gained importance. In this study, optically active chiral secondary alcohols which are used in various fields in the industry have been obtained by biotransformation of prochiral ketones. Four different lactic acid bacteria strains were used as biocatalyst in asymmetric reduction of prochiral ketones. Among these strains, Weissella paramesenteroides N7 was found to be the most suitable biocatalyst for the reduction of alcohols to ketones. The reaction conditions are systematically optimized for this biocatalysis with high enantioselectivity and conversion for the reducing agent. Asymmetric reduction of 1- (4-methoxyphenyl) propan-1-one (1r) by Weissella paramesenteroides N7, (R) -1- (4-methoxyphenyl) propan-1-ol (2r)> 99% enantiomeric excess and% 94 yields. Weissella paramesenteroides show that N7 can be used as a biocatalyst to obtain chiral carbinol with excellent yield and enantioselectivity. This method requires a promising and alternative approach to the synthesis of biologically important secondary chiral alcohols by a light and environmentally friendly method.