Yeni bir kolorimetrik kan kalsiyum ölçüm yönteminin geliştirilmesi ve uygulanması

A new approach for measuring calcium in serum using colorimetry is introduced, which utilizes the o-Cresol Phthalein Complexone (CPC) method. This method replaces traditional ultraviolet-visible (UV-Vis) spectrometry with a more straightforward and cost-effective smartphone-based digital image colorimetric technique. The optimal conditions determined for this method include using the green color model as the most effective, maintaining a distance of 25 cm between the detection camera and the sample solution, and positioning the light source 3 cm from the sample solutions. Under the optimum conditions, the coefficient of determination was 0.9917 within a linear dynamic range up to 20 mg /dL. The precision of the method based on the percent relative standard deviation was below 5%. The detection limit (LOD) and quantitation limit (LOQ) were determined to be 0.46 mg/dL and 1.56 mg/dL, respectively, which are adequate for measuring calcium levels in serum. The method's accuracy was confirmed through validation with an independent experiment using a UV-Vis method. The two methods demonstrated strong statistical agreement, underscoring the reliability of the proposed approach.