Antiproliferative and apoptotic effects of proton pumpinhibitor lansoprazole on DU-145 prostate cancer cells

Cancer has been a major threat on human health since 20th century and is one of the most significant causes of death among people. Prostate cancer is the second most cause of death among elder people. In spite of early detection and new treatments, there is a need for improvements for detection, diagnosis and therapy of prostate cancer. Ion channel blockers are promising cancer drugs not only modulate tumor microenvironment and pH but also overcome chemotherapy resistance and slow or even prevent invasion or metastasis by different mechanisms. Lansoprazole is a proton pump inhibitor that blocks V-ATPase and H+/K+ ATPase channels on cells. In this study, we aim to discover antiproliferative and apoptotic effects of lansoprazole on Du-145 prostate cancer cell lines. To explore these features, MTT assay and trypan blue counting for cytotoxicity were performed. To evaluate Caspase-9 and PARP expression, Western Blotting was done. Apoptosis was analyzed by Annexin/PI flow cytometry. In conclusion, it could be stated that lansoprazole promotes a nonapoptotic caspase-independent cell death on Du-145 castration-resistant prostate cancer cells and suppresses cell proliferation and growth dynamics at 6 hours, at 10 and 100 micromolar doses. Keywords: Prostate Cancer, Ion Channels, Lansoprazole, Du-145