Böbrek kanserinde WAVE3 gen ekspresyonunun analizi

, xi + 60 pages Cancer is a disease distinguished by uncontrollable cell proliferation that can develop in almost any organ or tissue, such as the kidney. Kidney cancer is a complex illness. Multiple distinct subtypes of kidney cancer develop in the kidney's cells; these subtypes vary in their histology, clinical manifestations, therapeutic responses, and underlying genetic causes. The Wiskott-Aldrich syndrome protein family verprolin homologous protein 3 (WAVE3) gene, whose official name is WASP family member3 (WASF3), belongs to the WASP-WAVE family and is an actin cytoskeleton remodeling protein. WAVE3 is required for many regulatory functions in cells, including organizing cell shape, regulating actin-filament polymerization of the cell membrane, motility, migration, and cell metastasis. Furthermore, it is associated with cellular proliferation and cytokinesis. Nonetheless, WAVE3 has a major function in cancer cell invasion and metastasis. Thus, it acts as a metastasis-enhancing protein. This present investigation on the Turkish population is the first to examine the relationship between the WAVE3 gene and kidney cancer. The aim of present study was to determine the expression level of the WAVE3 gene in kidney cancer tissues relative to healthy tissues. The study involved 11 kidney cancer tissues and 11 healthy tissues from the same participants. The gene's expression level was measured by the quantitative real time polymerase chain reaction technique (qRT-PCR). According to the findings of the present study, there is no difference in WAVE3 gene expression in kidney cancerous tissues when compared with healthy tissues (fold change = 0.93), and regarding this finding, statistically, no significant correlation was found (p = 0.87). Accordingly, further studies with large samples are needed to analyze and clearly understand the WAVE3 expression level relationship to kidney cancer. Key Words: Kidney cancer, Real-Time PCR, WAVE 3.