Examination of candidate pamp's role playing in basal defense in wheat brown rust disease with proteomic approach

Wheat, barley and corn are crucial food for both Turkey and world population. The production of these cereal is affected by various biotic and abiotic stress conditions and miss-use of fields. Biotic factors affected the yield viral, bacterial or fungal pathogens. Vital fungal diseases for cereals are rust, headblight and mildew both in Turkey and in the world. Proteomics among several "omics" technologies has been used recently to unveil mechanisms of plant diseases and immune responses against to these diseases. So far a number of proteomics studies are placed in literature on cereals like barley, wheat and their interaction with pathogens. In plant proteomics researchs up to date, rice and arabidopsis are used as model plant and their response to their biotic enemies mostly Arabidopsis as a model is threatened by bacteria mainly. Since rust diseases threated Arabidopsis have not been known yet immune system behaviors against to these pathogens have not been known. In this study, it is aimed to identify candidate molecular patterns that play role in pathogen triggered immunity by pathogen or elicitors released by pathogen in brown rust disease in wheat. Surface proteins were resolved in 2D-PAGE and 194 high abundant protein spots were digested with tyripsin, tyriptic peptides of each spot were analysed and identified by LC-MS/MS. Totally 8 proteins were predicted as effector candidates. PTTG_06852 and PTTG_06073 proteins targeted to mitocondria and chloroplasts respectively and having transit peptide effector candidates. 35% of identified proteins were localised in cytosol and 29% of them were locolasied in mitochondria.