Effects of various plant growth regulators on rooting of black elderberry (Sambucus nigra L.)

Sambucus nigra L. (Elderberry) is a grape-like perennial herb in the form of small tree or shrub belonging to Adoxaceae family used in the treatment of respiratory problems and asthma in folk medicine, as well as for food consumption. An efficient in vitro culture protocol of S. nigra as developed via multiple shoot formation. Shoot tip explants from naturally growing plants were cultured on MS media containing different concentrations and combinations of various plant groth regulators and metatopolin (mT). Shoot formation was obtained from all combinations used except the control medium. The regeneration efficiency was found to be higher in 1.0 mg/l BA + 0.1 or 0.5 mg/l IBA and 0.5 mg/l mT + 0.1 or 0.5 mg/l IBA ere also effective for shoot regeneration. When all combinations were compared in terms of shoot length and appearance of regenerants, the best medium was obtained with KIN in combination with IAA or IBA. Regenerated shoot were rooted on MS medium containing different concentartions of IAA, IBA or NAA. The best root formation was obtained with 0.5 mg/l NAA and 0.1 mg/l IBA, respectively. Compairing rooting medium for root length, 0.1 mg/l IBA medium was more effective. After, rooting, the plantlets were transferred to soil for hardening.