Investigation of the protective effects of alfa lipoic acid on rats given diethylnitrosamine

2SUMMARYThis study aimed to determine toxic effects of diethylnitrosamine on liver and to investigatethe protective effect of α-lipoic acid to oxidative stres related with the toxic effects ofdiethylnitrosamine.As material, nearly 3 month old 260±10.5 gr, 49 Spraque-Dawley kind male rats have beenused. The rats were equally divided into seven groups, group 1 (control), group 2(intraperitoneally one dose of 150 mg/kg diethylnitrosamine has been given), group 3(intraperitoneally one dose of 150 mg/kg diethylnitrosamine and 2 cc. olive oil with oralgavaj from 14 days has been given), group 4 (intraperitoneally one dose of 150 mg/kgdiethylnitrosamine and with oral gavaj 100 mg/kg/day α-lipoic acid by solving it in 2 cc. oliveoil for 7 days has been given), group 5 (intraperitoneally one dose of 150 mg/kgdiethylnitrosamine and with oral gavaj 100 mg/kg/day α-lipoic acid by solving it in 2 cc.olive oil for 14 days has been given), group 6 ( with oral gavaj 100 mg/kg/day α-lipoic acidby solving it in 2 cc. olive oil for 14 days has been given)and group 7 (with oral gavaj 2 cc.olive oil for 14 days has been given each day). At the end of the 15 days study ınderanesthesia, blood samples were taken from their hearts which anesthesia to the heparin tubes.Some quantity of the blood samples were kept as whole blood. The rest of the blood werecentrifugal, and their serums were gained. In the whole blood, GSH and MDA classifications,in the serums AST,ALT, GGT,ALP and ADA classifications have been conducted.Compared to the control group, in the group given diethylnitrosamine a considerabledecrease has been identified in the GSH level and the considerable increase in MDA, AST,ALT, GGT, ALP and ADA levels have been identified. It is identified that giving α- lipoicacid for both 7 and 14 days are protective, but 14 days of usage is more effective than 7 daysof usage.As a result it has been deducted that diethylnitrosamine depending on the toxic effects canenhance the oxidative stres, but α- lipoic acid prevents the lipid peroxidation occured againstthe stress and increases the levels of antioxidan molecules, such as reduced glutathioneand it can make a protective effect and playing role like scavenging of free radicals. In termsof this it is thought that it can be used to cure liver injuries because of alcohol, NSAImedicines, heavy metal poisoning, viral hepatitis and cancer. Besides as most of themedicines are metabolised from the liver, it is believed that it can be given as protector to thechronic ilnesses that long-term medicines must be used.Key Words: α-Lipoic acid, Adenosine deaminase, Diethylnitrosamine, Liver function tests,Malondialdehyde, Reduced glutathione.