The effect of quercetin on erythrocyte glucose -6- phosphate dehydrogenase (G6PD) enzyme activity in ethanol treated rats

The toxic effects of ethanol on human body are well known. In this study, we aimed to evaluate the effects of ethanol on erythrocyte (G-6-P-D) enzyme activity and the effects of quercetin, a number of antioxidant flavonoids on erythrocyte G-6-P-D activity in the recovery of the effects of ethanol. It?s well known that, during ethanol metabolism, the formation of reactive oxygen species (ROS) is induced and these agents damage the erythrocytes which are lack as nucleus and mitochondria. G-6-P-D catalyzes the key step in penthose phosphate pathway, during which NADPH molecules are produceds. NADPH is used to reduce the oxidized glutathione formed during detoxification reactions. In case of NADPH depletion, emolysis occurs as a result of lass of membrane integrity by inhibiting the ROS- induced lipid peroxidation. In this study, 25 male Sprague-Dawley rats weighing 280-320 g (3-3,5 months) were assiggned into one of the four groups. The rats were on Standard diet and drink water ad libitum. 12 h of light and dark aycles were performed and the environmental temperature was kept between 25-28 0C. The control group (n = 6) received saline i. g (2 ml/day). The quercetin group (n = 7) received quercetin once upon days 100 mg/ kg via i.g. and saline (1 ml/day) coute for 30 days. The Alcohol Group (n = 7) received ethanol ( %80 v/v, 1 ml/ day) via i. g. and saline (1 lm/day) Coute for 30 days. The Alcohol + Quercetin Group ( n = 5) received 1 ml of Ethanol ( %80 v/v) 2 hours after quercetin treatment once upon days 100 mg/ kg for 30 days. the blood was drawn on Lithium heparine coutaining tubes. After erythrocyte package was prepared, erythrocyte G-6-P-D activity was measured according to modified Zinkham Method. G-6-P-D activity was found to be higher in the Quercetin Group than those in the Alcohol Group ( 6,38 ± 0,4 IU/gHb vs 3,82 ± 0,67 IU/gHb, p<0,001). In the Alcohol Group, the erythrocyte G-6-P-D activity was found to be significantly decreased than those in the Control Group ( 3,82 ± 0,67 IU/gHb vs 6,03PDF created with pdfFactory trial version www.pdffactory.com± 0,4 IU/gHb, p<0,001). No statistically significant differences were found betweenthe Control Group and the Alcohol + Quercetin Group. Moreover, statisticallysignificant differences were observed in erythrocyte G-6-P-D activity between theAlcohol Group and the Alcohol + Quercetin Group ( 3,82 ± 0,67 IU/gHb ve 5,46 ±0,78 IU/gHb, p<0,001).As a couclusion, our results demonstrate that ethanol decrease, erythrocyteG6PD activity and quercetin was found to be beneficial in the prevetion of toxiceffect raised by ethanol.Key Words : Erythrocyte, ethanol, G6PD, oxidative stress, quercetinPDF created with pdfFactory trial version www.pdffactory.com