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In vitro bulblet production of different muscari species

2008
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Advisor: Prof. Dr. Fatma Ünal

Abstract (EN)

M. muscarimi (endemic), M. macrocarpum, M. neglectum and M. adilii (endemic) were studied in this thesis. Explants obtained from different species showed variable behavior; twin scales, twin scales from in vitro regenerated bulblets and lower portions of leaves were cultured on MS medium containing different concentrations of BAP-NAA, KIN-NAA and TDZ-NAA. The highest number of 19 bulbs were regenerated on MS medium containing 4 mg/l BAP-2mg/l NAA in M. muscarimi. The largest bulbs of 1,24 cm diameter were recorded on MS medium containing 1 mg/l BAP?1 mg/l NAA. The maximum number of 6 bulbs were noted on MS medium containing 2 mg/l Kinetin-0,5 mg/l NAA in M. macrocarpum. The largest bulbs with 1,39 cm diameter were recorded on the bulblets cultured on the MS medium containing 4 mg/l Kinetin-2 mg/l NAA and transferred to MS medium. The highest number of 8,25 bulbs were recorded on 0,1 mg/l TDZ-0,5 mg/l NAA in M. neglectum. However, the largest bulbs with 0,47-0,48 cm diameter were recorded on MS medium containing 0,15 mg/l TDZ- 0,5 and 2 mg/l NAA. Maximum number of 15,75 bulblets were regenerated on MS medium containing 4 mg/l BAP- 0,5 mg/l NAA in M. adilii. The maximum diameter of 0,58-0,54 cm was noted down on MS medium containing 1 mg/l BAP-1 AND 2mg/l NAA. M. muscarimi bulbs were rooted on MS medium containing 1 mg/l IBA. The bulbs of other three species were rooted on MS medium. It is expected that the results of this study will help in increasing the production speed of the plants.

Author

Çiğdem Alev Özel

How to Cite

Çiğdem Alev Özel (Doctorate thesis). In vitro bulblet production of different muscari species, 2008, Gazi University.

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